A Preliminary Study -September 2024
Dr Graham Exelby
Brainstem hypoperfusion, coat hanger pain and PEM have similar pathogenesis centered around hypoperfusion, and these are found in that same group of medical conditions linked by fatigue and cognitive impairment- ME/CFS, POTS, Fibromyalgia Syndrome, Gulf War Syndrome and Long COVID. The brainstem, which consists of the midbrain, pons and medulla, has been implicated in ME/CIFS in many studies. This region regulates the respiratory, cardiovascular, gastrointestinal, and neurological processes, which can be affected by long-COVID, Fibromyalgia syndrome, POTS, migraine and ME/CFS.
Research into coat hanger pain has provided pathways demonstrating where hypoperfusion induces muscle depolarization, weakness, and mitochondrial dysfunction. ME/CFS patients have reduced reserves of Adenosine triphosphate (ATP) vital for mitochondrial energy production, and replenishment of ATP may take days. In post exertional malaise (PEM) the metabolic dysfunction induced by the hypoperfusion moves the body into catabolic metabolism with increased “burn off” of amino acids, essential minerals and vitamins. Assessing amino acids may provide a picture of the metabolic dysfunction that characterizes the fatigue.
The hypoxia is believed to impair the function of enzyme pyruvate dehydrogenase (PDH) in ME/CFS causing changes in the vital Krebs/ Citric acid cycle responsible for our mitochondrial energy source leading to inadequate adenosine triphosphate (ATP) generation by oxidative phosphorylation and excessive lactate generation on exertion.(1) That there is also an inflammatory component may be seen in patient studies where the thromboinflammatory pathway of COVID may be associated with brainstem hypoperfusion and cerebral hyperperfusion, and where central sleep apnoea secondary to the hypoperfusion may be modulated by corticosteroids, and noted by Hoel, Fluge et al (2) in one of the metabolic ME/CFS subtypes.
Research by Fluge et al (1) may to allow us to see which amino acids are being “burnt off” in the body’s quest to maintain ATP when the PDH pathway is impaired. Abnormalities in aspartic acid /glutamate levels may provide a link to the hypermetabolic changes seen in our brain SPECT scans, while abnormal ethanolamine may reflect dysfunction in the phospholipid pathway seen in COVID-19 research. Hoel, Fluge et al (2) described 3 emergent subtypes, but with a complexity far more than their findings, the preliminary findings in amino acid dysfunction in ME/CFS and post-exertional malaise (PEM) linked also to phospholipid dysfunction that fits with the POTS and Long COVID DNA findings by Dr Valerio Vittone.(3)
Assays of neuroexcitatory amino acids glutamate, aspartic acid and histidine may provide explanations for the characteristic sensitization found in these conditions. Glutamate metabolism is complex and interacts with many other biochemical pathways. The specific amino acid profiles found in these studies may provide clues about the nature of the dysfunction, potentially guiding treatment approaches.
The preliminary studies have been hampered by inadequate numbers of “control” testing of patients who have recovered from their POTS and Long COVID. Those tested appear to demonstrate similar patterns of amino acid dysfunction as those who are symptomatic, implying an underlying metabolic disorder that may be associated with their individual DNA patterns impacted by the various infective and other “activators” and “drivers” found in the individual’s POTS, Long COVID and Fibromyalgia investigation.
Simply supplementing the low amino acids may be fraught with potential risks as it does not consider the very complex dysfunctional metabolisms that are occurring, and which require a more determined assessment. I believe these preliminary studies will foster a more detailed research that affects ME/CFS, POTS, Fibromyalgia as well as Long COVID and lay the path for improved management protocols.
Chronic Fatigue and Amino Acid Dysfunction-combining research from Fluge et al (1) and Hoel, Fluge et al (2)
Cort Johnson (4) in his 2021 paper described the findings of a large Norwegian study (2) by Hoel, Fluge et al, which looked at the metabolic changes that occur in Chronic Fatigue Syndrome (ME/CFS). They found 67 metabolites affected in CFS suggesting a core energy dysfunction, with widespread changes in lipids and amino acids. This was described as the cells trying to access energy any way they can, turning to metabolism of fatty acids and amino acids instead of carbohydrates.
Brand et al (5) described the classic role of mitochondria as oxidative phosphorylation, which generates adenosine triphosphate (ATP) by utilizing the energy released during the oxidation of the food we eat. ATP is used in turn as the primary energy source for most biochemical and physiological processes, such as growth, movement and homeostasis. We turn over approximately our own body weight in ATP each day, and almost all of this is generated by mitochondria, primarily within muscle, brain, liver, heart and gastrointestinal tract.
The pre-eminent role of eating is to provide the fuel for mitochondria, and breathing is to provide the oxygen and to remove the carbon dioxide produced during oxidative phosphorylation by mitochondria. Similarly, a major role of the cardiovascular system is to deliver the substrates (glucose, fatty acids, oxygen) and remove the products (carbon dioxide) of mitochondrial activity. While the functions of mitochondria include oxidative phosphorylation to produce cellular ATP, but they also have important roles in ion homeostasis, several metabolic pathways, apoptosis and programmed cell death, and in ROS production and consumption. All of these functions may be significant in ageing and/or disease. Damage may cause mitochondria to accumulate dysfunctional components.(5)
Figure 1. Proposed mechanism of ME/CFS linked to amino acid catabolism.
Category I: amino acids are converted to pyruvate, and therefore depend on PDH to be further oxidized. These are alanine (Ala), cysteine (Cys), glycine (Gly), serine (Ser), and threonine (Thr).
Category II: amino acids that enter the oxidation pathway as acetyl-CoA, which directly and independently of PDH fuels the TCA cycle for degradation to CO2. These are isoleucine (Ile), leucine
(Leu), lysine (Lys), Phe, tryptophan (Trp), and tyrosine (Tyr).
Category III consists of amino acids that are converted to TCA cycle intermediates, thereby replenishing and supporting the metabolic capacity of the cycle- histidine (His), and proline (Pro)
The asterisks indicate the amino acids significantly reduced in ME/CFS patients.
Source: Fluge et al., Metabolic profiling indicates impaired pyruvate dehydrogenase function in myalgic encephalopathy/chronic fatigue syndrome. JCI Insight. 2016 Dec 22;1(21):e89376. doi: 10.1172/jci.insight.89376. PMID: 28018972; PMCID: PMC5161229.(1)
Hoel, Fluge et al (2) found 3 subsets of patients in ME/CFS with metabolic patterns similar to those found in starvation and after intensive exercise. :
· High degree of fatty acid and amino acid breakdown with high levels of ketone derivatives suggesting a ketogenic slant in 40%. Reduced levels of amino acid metabolites and low tryptophan was found. With little evidence of mitochondrial dysfunction this pattern was similar to starvation and after high-energy exercise.
· Increased fatty acid breakdown but characterized by increased amino acid breakdown and evidence of mitochondrial dysfunction in 45%. There were increased pyruvate levels suggesting this wasn’t being broken down properly and that the mitochondria may not be getting the acetyl-CoA and NADH they needed. Increased tryptophan but lack of tryptophan derivatives suggested it wasn’t being broken down correctly. This group with the most severe symptoms, had metabolomic profiles similar to those of inflammatory diseases.
· The 3rd group, 15%, had intermediate profiles.
Hoel, Fluge et al (2) suggested that elevated energy strain may result from exertion-triggered tissue hypoxia and lead to systemic metabolic adaptation and compensation. Through various mechanisms, such metabolic dysfunction represents a likely mediator of key symptoms in ME/CFS.
Pyruvate Dehydrogenase and Acetyl-Coenzyme A ( Acetyl-CoA)
The impairment of pyruvate dehydrogenase (PDH) in ME/CFS is believed to be linked to the increased expression of pyruvate dehydrogenase kinases, specifically PDK1, PDK2, and PDK4. These inhibit PDH activity- the aberrant increase in PDK expression might be influenced by the activity of PPAR transcription factors, leading to impaired PDH function in ME/CFS patients.(1) This impairment in PDH results in obstructed pyruvate catabolism, forcing the body to rely more on acetyl-CoA-producing amino acids as alternative substrates for aerobic metabolism via the TCA cycle.
Dysfunction in this pathway has been suggested to be part of the brain fog in Long COVID, ME/CFS and POTS. The positioning of pyruvate can be demonstrated below at the keystone of the Citric Acid Cycle. (Figure 1 and 2)
Several factors can lead to increased expression of PDK1, PDK2, and PDK4:
· Hypoxia - Hypoxia-inducible factor (HIF), especially HIF-1α, activates the expression of PDK1 and PDK3 under low oxygen conditions (6)
· Malignancy- Cancer cells often have mutations that activate HIF signaling, leading to high PDK expression (6)
· Metabolic abnormalities- diabetes, insulin resistance, fasting and high-fat diets can upregulate PDK4 expression (6)(7)
· Various transcription factors (6)
· Hormones and nutritional factors- In starvation and diabetes, nutritional factors and hormones regulate PDK2 and PDK4 expression (6)
· Inflammation - Inflammatory conditions like diabetic nephropathy, sepsis, and amyotrophic lateral sclerosis correlate with increased PDK4 expression (7)
· Mitochondrial dysfunction - Impaired mitochondrial function can lead to compensatory upregulation of PDK (1)
· Specific to ME/CFS- increased expression of PDK1, PDK2, and PDK4 is observed, possibly due to aberrant regulation mechanisms involving PPARs and SIRT4. (8)
Acetyl-CoA is a metabolic intermediate that is involved in many metabolic pathways. It is produced during the breakdown of glucose, fatty acids and amino acids, and is used in the synthesis of many other biomolecules including fatty acids, cholesterol and ketone bodies. It is a key molecule in the Kreb’s, or Citric acid cycle, a series of chemical reactions that occur in the mitochondria and is responsible for generating energy in the form of ATP. (Figure 2)
Figure 2: Citric Acid Cycle (or Kreb’s cycle or TCA cycle)
Source: Narayanese (original file), WikiUserPedia (SVG), CC BY-SA 3.0 <http://creativecommons.org/licenses/by-sa/3.0/>, via Wikimedia Commons
Brainstem Hypoperfusion
The brainstem, which consists of the midbrain, pons and medulla, has been implicated in ME/CIFS in many studies. It regulates the respiratory, cardiovascular, gastrointestinal, and neurological processes, which can be affected by long-COVID and similar disorders eg migraine and ME/CFS.
Griffith University showed in 2023 that the brainstem is larger in Long Covid and ME/CFS patients (9) and the brainstem demonstrates an imbalance of neurochemicals in this same group in 2024. (10) These changes correlate with the SPECT scan findings in ME/CFS, POTS and Long COVID found in our studies.
The brainstem hypoperfusion also seen in SPECT scans from our clinic findings, is believed to be part of the same process of hypoperfusion and mitochondrial dysfunction that underpins the coat hanger pain of FMS and POTS.
In severe ME/CFS, Wirth et al (8) noted the reduction in blood flow in the brainstem from lying to sitting was 24.5%. They described the reduced blood flow to the brainstem causing neurological symptoms including impaired cognitive function or “brain fog.” This hypoperfusion can impact both the global and local regulation of blood flow in the brain. COVID-19 seriously affects the endothelium and there is evidence of chronic endothelial dysfunction in the post-Covid-syndrome similar to that in ME/CFS.(11)
They also reported an increase in intracranial pressure observed in ME/CFS patients, and later proposed by Hulens (12) and Bragee (13) also has been frequently observed compounding problems. The combination of brain perfusion abnormality and intracranial CSF dysfunction has been frequently observed in our clinic, compounding problems and intricately linking the 2.
As a possible explanation for the orthostatic intolerance and the decrease in cerebral blood flow they proposed the presence of both a strong vasoconstrictor effect mediated by an elevated sympathetic tone and weakened vasodilator influences. Clinic findings confirm this is a significant component, but we have also found mechanical causes.- Brainstem Hypoperfusion, Coat Hanger pain and Post-Exertional Malaise in POTS and Long COVID. (14)
Wirth et al (8) also reported muscle mitochondrial dysfunction, as evidenced by higher levels of pyruvate and lower levels of ATP and phosphocreatine in muscles, suggesting an impairment in muscle energy metabolism, which is also observed in ME/CFS, indicating a likely overlap in the pathophysiological mechanisms of these conditions.
Long Covid research by Appleman et al, (15) has also shown post-exertional malaise (PEM), with associated fatigue, pain and local and systemic metabolic disturbances, severe exercise-induced myopathy and tissue infiltration of amyloid-containing deposits in skeletal muscles of patients with long COVID.
The impaired glymphatic function seen in Long COVID with its impairment of the paravascular space function also appears to have far-reaching effects on symptoms such as fatigue, brain fog and head pressure by CSF flow dysfunction.
Combining the research into the Glymphatic dysfunction, Intracranial Hypertension by Hulens (11), Bragee (12) and others with brainstem hypoperfusion and the catabolic metabolism of coat hanger pain and PEM provides a satisfactory hypothesis for much of the symptomatology in all of these.
The use of brain SPECT scans in POTS, Long COVID and ME/CIFS has demonstrated brainstem hypoperfusion which may assist us in determining whether we may be dealing with mitochondrial dysfunction eg after Infectious Mononucleosis, or a vascular/autonomic dysfunction with brainstem hypoperfusion with the sequalae from this, or more commonly, a combination of both. - Brainstem Hypoperfusion, Coat Hanger pain and Post-Exertional Malaise in POTS and Long COVID. (14)
Post-exertional malaise (PEM)
Post-exertional malaise (PEM) has been compared to the metabolic changes that a marathon runner may experience following their race. Coat hanger pain can contribute to PEM through hypoperfusion and muscle ischaemia. When muscles have inadequate oxygen then switch to anaerobic metabolism, with accumulation of lactic acid which causes the characteristic cramping and pain. The relationship between the two is shown with exercise, where in POTS even minor exertion increases pain, fatigue and cognitive difficulties which characterize PEM. (16) The interplay between reduced blood flow, lactic acid accumulation and autonomic dysfunction leads to a snowballing increase in symptoms and prolonged recovery times. ME/CFS patients have reduced reserves of Adenosine triphosphate (ATP) vital for mitochondrial energy production, and replenishment of ATP may take days.
PEM in ME/CFS involves complex metabolic changes including alterations in amino acid metabolism, with evidence of affects 24 hours after exercise and PEM activation. Non-essential amino acids, particularly those that can fuel the TCA cycle independently of pyruvate dehydrogenase (whose function is impaired in PEM) may become increasingly important for maintaining energy production during PEM episodes,(17(18(19) as shown by Fluge et al.(1)
Hypermetabolism appears to play a significant role in PEM characterized by increased excretion of urine metabolities indicating an abnormally high rate of metabolism. The hypermetabolism in PEM is also associated with:
the increased excretion of methylhistidine in urine during PEM indicates elevated muscle protein breakdown. This may also contribute to amino acid depletion as muscle is a significant reservoir of amino acids in the body. (1)
intestinal barrier breakdown,
acetate excretion,
glycolytic abnormalities with altered glucose: lactate ratios,
purine metabolism dysregulation with decreased hypoxanthine,
hypoacetylation affecting multiple cytoplasmic enzymes and DNA histone regulation affecting cellular function and gene expression
metabolite loss most likely from hypermetabolic state and hypoacetylation contributing to the prolonged recovery period in CFS patients after exercise.
Potential Causes of Fatigue in Long COVID
COVID has enabled researchers to look more closely at pathogenesis of many of the problems seen in both POTS and Long COVID. While these are being seen in COVID, basic principles apply in other causes. For many, knowledge of whether there is brainstem hypoperfusion (reduced blood flow), or abnormal amino acids/neurotransmitters can help decide a pathway to tackle the fatigue.
Table 1: Potential Causes of Fatigue in Long COVID- combining available and ongoing clinic research
· Reactivation of EBV and similar viruses that may cause mitochondrial dysfunction in the first place
Mitochondrial dysfunction, as evidenced by higher levels of pyruvate and lower levels of ATP and phosphocreatine in muscles (8)
Brainstem hypoperfusion with mitochondrial dysfunction /oxidative stress (CFS and coat hanger pain with progressive muscle depolarization) and catabolic metabolism /amino acid dysfunction with impact on ATP production.(20)(21)
Astrocyte /glymphatic dysfunction (TLR2-driven or crosstalk with microglia/mast cells)
Neurotransmitter dysfunction -Dopamine/serotonin/glutamate pathways and amino acid dysfunction (20)
Intracranial pressure abnormality with HPA axis dysfunction (14)(22)(23)
Direct cardiac damage (eg pericarditis, myocarditis, reduced Ejection Fraction)
Pulmonary damage- embolic, inflammatory, malignancy
Renal dysfunction through multiple causes including renal vein thrombosis and nephrotic syndrome
Mitochondrial dysfunction /oxidative stress. There are mitochondrial and inflammatory deleterious gene variants that are critical to mitochondrial dysfunction and the exaggerated immune response caused by COVID (3)
Metabolic pathway abnormality/ DNA mutations /dietary triggers. These include PEMT mutations and dysfunction in phosphatidycholine and phosphatidylserine metabolism (3)(23)(24)
Other vital DNA mutations associated with dysfunction include APO E4 with its known increased cardiovascular, neurodegenerative and liver dysfunction potential.(3)
Liver disease, with reduced clearance of fibrin degradation products
Undiagnosed malignancy that can accompany Natural Killer Cell dysfunction.(21)(25)(26) It is though that NK cells are functioning at about 20% capacity in Long COVID, ME/CFS and GWS.(22) TLR4 activation can cause NFkB dysfunction and dysregulation of malignancy risk
Small fibre neuropathy
Autonomic instability eg POTS, dysautonomia, orthostatic hypotension
Impaired cardiac function
Very common (again IL-6 and TNFa)-accompanying exaggerated neuropathy, rheumatoid arthritis, reactive arthritis, PMR strongly suggestive of TLR4 and STAT3 mutations (3)
Cardiac preload failure (unexplained shortness of breath with postural change)- best described as failure of the priming of the “pump-” currently at research level.
Direct cardiac involvement (including fibrin dysfunction)
Locus coeruleus/ Nucleus Tractus Solitarius dysfunction
Dysfunctional baroreceptor signalling from the carotid baroreceptor and low pressure receptors in heart and great veins
Aberrant azygous anatomy/ sympathetic activation
Reduced blood flow back to heart with arms raised when venous TOS is present.
Figure 3: SPECT Scans showing brainstem hypoperfusion and hyperperfusion
These demonstrate the mixed hyperperfusion and brainstem hypoperfusion typical of POTS.
Green represents normal perfusion. The blue areas reflect hypoperfusion, green normal, yellow, red then white increased metabolic activity/ hyperperfusion. The hyperperfusion is thought to be from endotheiliitis associated with intracranial vascular pressure as there is increasing evidence from our studies of impaired venous return causing a “backup” of venous pressure. Stagnant blood may play a part with its known activation of inflammatory cytokines causing the endotheleiitis. The scan image itself probably reflects increased levels of excitotoxic neurotransmitters affecting the brain through penetration through the dysfunctional BBB from the dysfunctional venous return.
Source: Mermaid Molecular Imaging
Amino Acid Metabolism
There are 20 amino acids, organic compounds composed mainly of nitrogen, carbon, hydrogen, and oxygen. Animals have seven conditionally essential amino acids that can be synthesized and are usually not required in the diet. However, they are essential components of the diet for specific populations that cannot synthesize them in adequate amounts. There are four “nonessential” amino acids can be synthesized and, thus, are not required in diet. The remaining nine “essential” amino acids are obtained from diet.(27)
Amino acid metabolism refers to the biochemical pathways that produce, break down, and use amino acids. The body uses amino acids to make proteins, enzymes, hormones, and other important molecules. Protein synthesis and degradation are both essential for maintaining homeostasis in the body.(28)
Amino acids can also generate glucose, ATP, and fatty acids, and they are metabolic precursors for numerous biomolecules, including haeme groups, nucleotide bases, and signalling molecules (catecholamines, neurotransmitters). In addition, they are essential for epigenetic modifications.(27)
Amino acid oxidation is a process that helps the body release energy from protein molecules. This process occurs in the liver and muscles and results in the production of ketone bodies which can be used by cells to generate energy.(28)
Brief Guide to Amino Acids (27)
The citric acid cycle or Kreb’s cycle or TCA cycle (Figure 1) is a series of biochemical reactions to release the energy stored in nutrients through oxidation of acetyl-CoA derived from carbohydrates, fats and proteins. This energy released is in the form of ATP or Adenosine triphosphate, the “molecular unit of currency.” Glycolytic and TCA cycle intermediates generate the nonessential and conditionally essential amino acids.
Free amino acids produced from degradation of either cellular proteins or dietary proteins are deaminated to yield NH4+ and a carbon skeleton. NH4+ enters the urea cycle and the carbon skeleton can enter metabolic pathways to generate ATP, glucose, and fatty acids.
Glutamate acts as both a nitrogen donor and acceptor and is the central amino acid for the movement of nitrogen among amino acids.
Tyrosine is the precursor to generation of noradrenalin, adrenalin, dopamine, and melanins.
Methionine provides the methyl group for many DNA and histone methyltransferases to regulate epigenetics.
Cysteine, glutamate, and glycine generate the antioxidant glutathione.
Nitric oxide synthases use arginine to generate nitric oxide (NO).
Glycine and glutamate can serve as neurotransmitters.
Glutamate can generate another amino acid neurotransmitter, γ-aminobutyric acid (GABA), which does not participate in protein synthesis.
Tryptophan is a precursor for serotonin.
Serotonin is used to generate melatonin.
L dopa is converted to dopamine, by enzyme amino acid decarboxylase. This can occur in both dopaminergic and serotonergic neurons (29)
Tryptophan is converted to serotonin also involving enzyme amino acid decarboxylase.(29)
Histidine is converted to histamine, involving histidine decarboxylase
Phenylalanine is converted to phenylethylamine (PEA) by enzyme amino acid decarboxylase. This conversion is dependent on Vitamin B6 as a cofactor. This conversion can occur in various tissues including the brain, and is regulated by monoamine oxidase (MAO).(29)(30) Phenylethylamine (PEA) is a neurotransmitter and neuromodulator. It rapidly crosses the blood brain barrier but has a very short half-life in the brain, rapidly metabolized by monoamine oxidase M. (31)
It increases the release and inhibits the reuptake of dopamine and noradrenalin in the brain, leading to higher levels which can improve mood, focus and concentration.(28)
PEA activates trace amine-associated receptors further enhancing dopamine and serotonin release.(31)
By increasing dopamine PEA may improve working memory, executive function and attention, potentially helping ADHD
Increased dopamine and serotonin may improve mood and reduce anxiety and depression
L dopa and tryptophan are dietary derived. Amino acid decarboxylase is also B6 dependant.
Serotonin/ dopamine have an effect on glutamate through the DARPP-32 pathways. This is a complicated pathway involving multiple steps.
DARPP-32 is involved in the modulation of glutamatergic signalling by dopamine and serotonin, and is an integrator of various different enzymes and regulatory mechanisms.
The Essential Amino Acids (32)
Phenylalanine: Your body turns this amino acid into the neurotransmitters tyrosine, dopamine, epinephrine, and norepinephrine. It plays an integral role in the structure and function of proteins and enzymes and the production of other amino acids.
Valine: This is one of three branched-chain amino acids (BCAAs) on this list. helps stimulate muscle growth and regeneration and is involved in energy production.
Threonine: This is a principal part of structural proteins such as collagen and elastin, which are important components of your skin and connective tissue. It also plays a role in fat metabolism and immune function.
Tryptophan: Often associated with drowsiness, tryptophan is a precursor to serotonin, a neurotransmitter that regulates your appetite, sleep, and mood.
Methionine: This amino acid plays an important role in metabolism and detoxification. It’s also necessary for tissue growth and the absorption of zinc and selenium, minerals that are vital to your health.
Leucine: Like valine, leucine is a BCAA that is critical for protein synthesis and muscle repair. It also helps regulate blood sugar levels, stimulates wound healing, and produces growth hormones.
Isoleucine: The last of the three BCAAs, isoleucine is involved in muscle metabolism and is heavily concentrated in muscle tissue. It’s also important for immune function, haemoglobin production, and energy regulation.
Lysine: Lysine plays major roles in protein synthesis, calcium absorption, and the production of hormones and enzymes. It’s also important for energy production, immune function, and collagen and elastin production.
Histidine: Your body uses this amino acid to produce histamine, a neurotransmitter that is vital to immune response, digestion, sexual function, and sleep-wake cycles. It’s critical for maintaining the myelin sheath, a protective barrier that surrounds your nerve cells. (32) The metabolism of histidine, glutamine and glutamate are closely related, so imbalances could disrupt normal metabolic processes. Histidine can be degraded to glutamate via urocanic acid.(33) It has a role in carnosine synthesis which may play a part in PEM. (34)
Non-essential amino acids include (35)(36)
1. Alanine clears toxins released during muscle protein breakdown, aids in regulating blood glucose and cholesterol, serves as an energy source for muscles and the CNS and assist in lymphocyte production
2. Arginine is a precursor for nitric oxide, accelerated wound healing, renal detoxification, hormone homeostasis, supports immune system function
3. Asparagine is important for neuron development and CNS homeostasis, helps build connective tissue and aids generation of digestion with generation of secretions and mucus in the GI tract.
4. Aspartic acid is an excitatory neurotransmitter and is involved in synthesis of other amino acids including methionine, threonine, isoleucine and lysine. It plays a role in the citric acid cycle for energy production and the urea cycle. It acts as an excitatory neurotransmitter in the ventral spinal cord similar to glycine (which is inhibitory).(37) Aspartate and glutamate can excite virtually every neuron in the CNS at low concentrations.(38) Aspartate is found in large quantities in neuroendocrine tissues eg hypothalamus. (39) Aspartate and glycine form an excitatory/inhibitory pain in the spinal cord, similar to glutamate/GABA in the brain.(37)
5. Cysteine stimulates collagen production and is involved in the synthesis of glutathione, and plays a role in protein folding and stability
6. Glutamic acid (the anionic form is known as glutamate) is an α-amino acid that is used by almost all living beings in the biosynthesis of proteins. It is a non-essential nutrient for humans, meaning that the human body can synthesize enough for its use.-(discussed below)
7. Glutamine is the most abundant free amino acid in the body, providing nitrogen for synthesis of other amino acids, improves exercise endurance, supports lymphocyte/ immune function, is involved in glutathione synthesis, and is essential for intestinal cell metabolism
8. Glycine is involved in the production of DNA, phospholipids and collagen. It helps in the production of bile acids and supports glutathione synthesis. It acts as an inhibitory neurotransmitter in the CNS. Aspartate and glycine form an excitatory/inhibitory pain in the spinal cord, similar to glutamate/GABA in the brain.(37)
9. Proline is a major component of collagen, crucial for wound healing and tissue repair. It supports collagen function and structure and helps maintain skin elasticity.
10. Serine is involved in phospholipid formation, is a precursor of several amino acids including glycine and cysteine. It plays a role in DNA and RNA synthesis, and is important for proper functioning of the CNS.
11. Tyrosine is a precursor for neurotransmitters dopamine, noradrenalin and adrenalin. It is involved in melanin production, helps in synthesis of thyroid hormones, and supports functioning of the adrenal, thyroid and pituitary glands.
Preliminary Amino Acids Identified in “Burn Off”
Examination of amino acid profiles using plasma and urinary assay demonstrates a number of response patterns. Accuracy can depend on collection techniques and avoidance of amino acid supplements before testing. Elevated Taurine for example may be seen after consumption of energy drinks. Elevated glutamic acid and 1- and 3-methylhistidine have also been seen in the urine assays.
Elevated glycine may point to altered neurotransmitter balance, as glycine is an inhibitory neurotransmitter (1), or potential issues with detoxification processes as glycine is involved in various detoxification processes. (1)
Abnormal asparagine has been found in one study to correlate with gastrointestinal symptoms. Elevated asparagine is thought by researchers may reflect a chronic state of energy deficiency or hypometabolism.(2)(61)
Plasma assays have shown low threonine, tyrosine, arginine, likely to reflect prolonged PEM activity and overall reduced levels.
Low levels of urinary ethanolamine, lysine, tyrosine, histidine, aspartic acid and have been observed in our preliminary studies, often seen collectively. Ethanolamine in particular may have more relevance in the phospholipid pathway dysfunction, and may be involved in the thromboinflammatory processes seen in Long COVID with its association with PEMT mutations.
Glutamate, aspartic acid and histidine levels may reflect the neuroexcitatory changes that produce the characteristic sensitization changes seen in most patients, may reflect the hypermetabolic changes seen in brain SPECT scans, and be responsible for the brain fog of Functional Neurological Disorder.
High plasma branched-chain amino acids (BCAAs) eg isoleucine, leucine and valine appear indicative of several metabolic disturbances. Possible scenarios include this may reflect mitochondrial dysfunction, a response to the inflammatory processes occurring as these amino acids can modulate immune function,, and they also compete with other amino acids eg tryptophan and tyrosine for transport across the blood-brain barrier potentially affecting serotonin and dopamine synthesis. BCAAs play a role in muscle protein synthesis and energy production. Elevated levels could reflect the deconditioning that occurs in POTS.
Amino acids changes links to sensitization.
Elevated levels of glutamic acid with low aspartic acid also seen and may reflect the dysfunctional glutamate seen in Functional Neurological Disorder,(FND) but these may be at least in part collection errors in glutamatic acid/glutamine. The assay for Glutamate / Glutamic acid is often not reliable as glutamine decomposes to glutamate quickly and this can produce elevated levels dependent on collection technique.
Elevated plasma histidine may be an important component of PEM and the excitotoxicity.
In the normal glutamic acid, low aspartic acid and histidine pattern, this suggests there may be a disruption in metabolism of amino acids without directly affecting glutamate levels. Aspartic acid and glutamic acid are both excitatory neurotransmitters, so a decrease in aspartic acid may be a compensatory mechanism to maintain overall excitatory balance.(55) Low histidine could indicate altered histamine signalling, which interacts with the glutamatergic system.(56)
With elevated glutamic acid, this pattern directly reflects glutamate dysfunction, with excess glutamate potentially leading to excitotoxicity.(57) Elevated glutamate levels have been associated with various neurological and psychiatric conditions, including autism spectrum disorders.(58)
These differing profiles likely represent distinct underlying mechanisms of glutamate dysfunction:
The first pattern may indicate issues with amino acid transport or metabolism, affecting related neurotransmitter systems without directly elevating glutamate.
The second pattern suggests a more direct problem with glutamate regulation, either through increased production, decreased clearance, or altered receptor function.(59)
Amino Acid Dysfunction in catabolic “Burn Off”
Glutamate dysfunction has been identified in ME/CFS, Long COVID, Gulf War Syndrome, fibromyalgia, autism spectrum, ADHD, migraine, Alzheimer’s disease, Parkinson’s disease, and others. The neurotoxic changes from glutamate and other neuroexcitatory neurotransmitters identified in our amino acid studies appear to starting to answer many of the questions on the characteristic central sensitization that accompanies these conditions.
The convincing hypothesis of glutamate/astrocyte dysfunction by Guedl et al (40(41)) in Long COVID links neurotransmitter/amino acid neurotoxic changes with a variety of problems, notably Functional Neurological Disorder which is unfortunately thought of as a psychiatric problem, but reflects the damage caused by these neuroexcitatory/ neurotoxic amino acids, especially glutamate and aspartic acid following access through the BBB.
Examination of amino acid profiles using plasma and urinary assay demonstrates a number of response patterns. The neuroexcitatory amino acids glutamate and aspartic acid often complemented by low inhibitory glycine which may impact on increased neurotoxicity are frequently seen. The differences then provide a pathway to look at the metabolic differences that are occurring, and how these may be modulated. Excitatory amino acids like glutamate and aspartate can have neurotoxic effects when present in excessive amounts.
Trials of managing the known glutamate-associated conditions simply with low glutamate diets, especially in Gulf War Syndrome (GWS) veterans have proven to be largely ineffective, reflecting the complexity beyond the brief of the investigations. Most studies appeared to focus only on the glutamate pathways. By extending the investigations to include a broader range of amino acids (28) other neuroexcitatory dysfunction has been identified, eg glutamate and aspartic acid dysfunction implicated in autism spectrum disorder.(32)(35)
Our studies have also found dysfunction in aspartic acid, an excitatory amino acid neurotransmitter in the CNS, that stimulates NMDA receptors, though not as strongly as glutamate. Studies in 1960’s and 1970s in animal studies showed that oral intake of glutamate or aspartate could cause neuronal cell death in brain regions lacking a blood-brain-barrier (BBB).(42) This we believe becomes critical in the brain SPECT scans with the cerebral hyperperfusion reflecting endothelial dysfunction, disruption of the BBB and entry of the neuroexcitatory amino acid neurotransmitters into the brain seen in ME/CFS, POTS, and Long COVID.
The neurotoxic effects of neuroexcitatory amino acids like glutamate and aspartic acid appear to be mediated through excessive activation of glutamate receptors, especially NMDA receptors, leading to increased calcium influx and subsequent neuronal damage. Nitric oxide (NO) also appears to play a role.(42)
The NMDA (N-methyl-D-aspartate) receptor pathway, a glutamate and ion channel protein receptor that is activated when glycine and glutamate bind to it, is integral to excitatory neurotransmission in the brain, and its function can be influenced by various factors, including mutations in ion channels like TRP (transient receptor potential) channels. Mutations in TRP channels, as explored by Prof Marshall-Gradisnik and her team at National Centre for Neuroimmunology and Emerging Diseases (NCNED), Griffith University, can alter their function, potentially affecting calcium influx and homeostasis.
The team’s publications focussed primarily on the role of TRPM3 (Transient Receptor Potential Melastatin 3) ion channels in ME/CFS. They have demonstrated that TRPM3 channel activity is impaired in ME/CFS patients, particularly in Natural Killer (NK) cells. The dysfunction of TRPM3 channels affects calcium influx into cells, which is crucial for various cellular processes, including the function of NK cells. Their research has explored potential therapeutic interventions, including the use of low-dose naltrexone (LDN) to potentially improve TRPM3 function in ME/CFS patients.
There are potential approaches to mitigate aspartic acid neurotoxicity with dietary modification removing processed food containing aspartic acid, using antioxidants, phytochemicals such as curcumin and quercetin.(43)
The amino acid changes of catabolic dysfunction provide a potential pathway for management by metabolic profiling, particularly when the physical and other “drivers” in these conditions are identified and managed. The impact of diet and metabolic management is so very important as avoiding food the body sees as a threat reduces the cytokine response that drives the inflammation further, while simple treatments such as walking, improved posture and physical rehabilitation improves lymphatic flow and reduction of POTS symptoms.
There are differences from our amino acid findings and those found by Fluge et al (1), we believe as our focus has been on the range including POTS, Fibromyalgia Syndrome and Long COVID, where chronic fatigue is an active component, rather than just ME/CFS, which was their focus of the major research, so when combined with the DNA findings by Dr Vittone (3), dysfunctional metabolic pathways emerge.
Neuroexcitatory amino acids:
Glutamate: Glutamate pathways comprise the major excitatory system in the brain and linked to many other neurotransmitter pathways, including GABA, the main inhibitory neurotransmitter. Glutamic acid (the anionic form is known as glutamate) is an α-amino acid that is used by almost all living beings in the biosynthesis of proteins. It is a non-essential nutrient for humans, meaning that the human body can synthesize enough for its use.-(discussed below) It is found in high concentrations throughout the brain and compartmentalized in synaptic vesicles for Ca2+-dependent release. It is the primary excitatory neurotransmitter in the brain, responsible for fast excitatory transmission, synaptic plasticity, learning and memory processes and sensory perception.(23) Under certain conditions excessive activation of excitatory amino acid receptors can lead to neurotoxicity, or “excitotoxicity.”(42) Excitotoxicity can occur:
Excessive release, overwhelming the normal mechanisms for clearance and reuptake, where the exacerbated or prolonged activation of glutamate receptors starts a cascade of neurotoxicity that ultimately leads to the loss of neuronal function and cell death. The molecular mechanism that triggers excitotoxicity involves alterations in glutamate and calcium metabolism, dysfunction of glutamate transporters, and malfunction of glutamate receptors, particularly N-methyl-D-aspartic acid receptors (NMDAR).
Impaired glutamate-glutamine cycle, when the astrocytes fail to effectively recycle glutamate leads to its accumulation in the extracellular space. On the other hand, excitotoxicity can be regarded as a consequence of other cellular phenomena, such as mitochondrial dysfunction, physical neuronal damage, and oxidative stress.(44)
Aspartic acid is an excitatory neurotransmitter and is involved in synthesis of other amino acids including methionine, threonine, isoleucine and lysine. It plays a role in the citric acid cycle for energy production and the urea cycle. It acts as an excitatory neurotransmitter in the ventral spinal cord similar to glycine (which is inhibitory).(37) Aspartate and glutamate can excite virtually every neuron in the CNS at low concentrations.(38) Aspartate is found in large quantities in neuroendocrine tissues eg hypothalamus. (39) Aspartate and glycine form an excitatory/inhibitory pain in the spinal cord, similar to glutamate/GABA in the brain.(37)
Histidine is essential in protein synthesis, tissue repair and production of red and white blood cells.
It is synthesized in the tissue by carnosine synthase from histidine and β-alanine, at the expense of ATP hydrolysis.
It plays an important role in enzymes eg serine proteases (such as trypsin.)
Histidine is required as a precursor of carnosine in human muscle and parts of the brain where carnosine appears to play an important role as a buffer and antioxidant. Carnosine is synthesized from histidine and β-alanine, at the expense of ATP hydrolysis. (45)
Carnosine plays an important role in exercise performance and skeletal muscle homeostasis. It is widely used among athletes in the form of supplements. It may play a significant role in PEM, but at present this is merely speculative. (46)
Histidine can be decarboxylated to histamine by histidine decarboxylase. This reaction occurs in the enterochromaffin-like cells of the stomach, in the mast cells of the immune system, and in various regions of the brain where histamine may serve as a neurotransmitter.
Inborn errors have been recognized in all of the catabolic enzymes of histidine. Histidine is required as a precursor of carnosine in human muscle and parts of the brain where carnosine appears to play an important role as a buffer and antioxidant. It can also be degraded to glutamate.(45)
Low levels of Pl histidine have been identified in PEM in our amino acid studies, which suggests, when Pl glutamate levels are elevated, increased catabolism of histidine to glutamate.
Histidine supplementation can be inappropriate in liver disease, with increases in ammonia and glutamine.
The increasing glutamate levels may increase the glutamate excitotoxicity.(347
Ethanolamine and Phospholipid Dysfunction
As data levels progress, the convoluted implications of the altered amino acids are becoming apparent, in particular in excitatory neurotransmission, and collagen formation and integrity.
Research has found a complex relationship between phospholipid dysfunction and COVID-19 severity. COVID-19 coagulopathy shares pathophysiologic features with antiphospholipid syndrome (APS), including endothelial dysfunction, platelet activation, complement activation, and neutrophil extracellular trap formation. Butt, Erkan and Lee (48) describe: “Antiphospholipid antibody production in COVID-19 is common, with 50% of COVID-19 patients being positive for lupus anticoagulant in some studies, and with non-Sapporo criteria antiphospholipid antibodies being prevalent as well. The biological significance of antiphospholipid antibodies in COVID-19 is uncertain, as such antibodies are usually transient, and studies examining clinical outcomes in COVID-19 patients with and without antiphospholipid antibodies have yielded conflicting results.”
Anti-phosphatidylserine/prothrombin (aPS/PT) IgG antibodies were significantly higher in COVID-19 patients with neurological symptoms compared to controls. (44) Ethanolamine plays an important role in phospholipid metabolism, and its dysfunction has been associated with phospholipid abnormalities observed in COVID-19 patients.
Ethanolamine is a component of cell membranes and involved in lipid metabolism. It is a precursor for phosphatidylethanolamine synthesis to phosphatidylcholine.
PEMT mutations- Phosphatidylethanolamine N-methyltransferase (PEMT) catalyses phosphatidylcholine synthesis. PEMT and similar mutations are involved in vascular complications, neurodegeneration and thrombo-inflammation. It is thought that PEMT gene polymorphisms are associated with non-alcoholic fatty liver disease (NAFLD). Fatigue is a common symptom of PEMT mutations and its associated mitochondrial dysfunction, and is thought to be involved in neurodegenerative disease. PEMT is involved in the biosynthesis of phosphatidylcholine (PC) from phosphatidylethanolamine (PE), and likely to be the underlying culprit in persistent D-Dimer tests in Long Covid. A mutation in PEMT could potentially lead to an accumulation of phosphatidylethanolamine, and by extension affect ethanolamine levels. PEMT is a critical DNA mutation in POTS and Long COVID. (3)
The association between PEMT mutations and thromboinflammatory processes, especially as seen in COVID may explain some of the POTS and Long COVID symptoms. Ramya Dwivedi (49) describes that “vascular complications in COVID-patients links phosphatidylserine (PS) to thrombo-inflammation. Thrombo-inflammation plays a critical role through complement activation and cytokine release, platelet overactivity, apoptosis (thrombocytopathy), as well as coagulation abnormalities (coagulopathy).”(49)
Rauchet al (50) found phosphatidylserine was associated with increased thrombo-inflammation and vascular complications. The DNA mutations found by Dr Valerio Vittone (3) have contributed significantly to understanding the involved pathways, particularly in patients with DNA mutations eg PEMT There are no known biomarkers for this mutation, so abnormalities in this amino acid metabolism may provide a valuable link and a way to monitor metabolism and abnormalities in this pathway.
PEMT mutations could contribute to oxidative stress by altering membrane composition and function. This could affect cellular signalling, ion channel function and vascular integrity
Abnormalities in amino acid metabolism involving ethanolamine could be both a cause and a consequence of the metabolic disturbances associated with PEMT mutations
Fluge (1) Category II:
Amino acids that enter the oxidation pathway as acetyl-CoA, which directly and independently of PDH fuels the TCA cycle for degradation to CO2.
Isoleucine (Ile) One of the three BCAAs, isoleucine is involved in muscle metabolism and is heavily concentrated in muscle tissue. It’s also important for immune function, haemoglobin production, and energy regulation.
Leucine (Leu) is a BCAA that is critical for protein synthesis and muscle repair. It also helps regulate blood sugar levels, stimulates wound healing, and produces growth hormones.(32)
Lysine (Lys) is vital for protein synthesis, hormone production and immune function. Low lysine levels may be caused by prolonged stress, too much arginine or histidine supplementation competing for absorption, or carnitine deficiency.
Symptoms may include muscle weakness, fatigue, or high serum triglycerides due to decreased fatty acid transport from low levels of carnitine and lysine.
Low utilization of lysine can lead to decreased hydroxylation, affecting the overall structure of collagen molecules, and making them more susceptible to degradation from mast cell-derived proteases, reducing overall collagen stability and strength.
Insufficient lysine modification can interfere with normal assembly of collagen fibrils, affecting the mechanical properties of tissues
As collagen is a major component of the extracellular matrix, this may lead to broader issues in tissue integrity. (51)(52)
Lysine is a building block for collagen synthesis, and low utilization may decrease overall collagen production
Nicotinamide (Vitamin B3) can influence lysine’s role in collagen synthesis, especially in the context of diabetes and wound healing. Lysine’s role in collagen synthesis is through hydroxylation which is facilitated by the conversion of NAD+ to NADH, which involves nicotinamide as a component of NAD+.(53)
Phenylalanine (Phe) Your body turns this amino acid into the neurotransmitters tyrosine, dopamine, epinephrine, and norepinephrine. It plays an integral role in the structure and function of proteins and enzymes and the production of other amino acids.(32)
Tryptophan (Trp) Often associated with drowsiness, tryptophan is a precursor to serotonin, a neurotransmitter that regulates your appetite, sleep, and mood.(32)
Tyrosine (Tyr) is a precursor for neurotransmitters dopamine, noradrenalin and adrenalin. It is involved in melanin production, helps in synthesis of thyroid hormones, and supports functioning of the adrenal, thyroid and pituitary glands.(32) Tyrosine dysfunction particularly due to tyrosine hydroxylase, affects the conversion of tyrosine to L-Dopa, the direct precursor of dopamine. Dysfunction in tyrosine hydroxylase can result in neurological symptoms eg dystonia, tremor and Parkinson’s disease.
Fluge (1) Category I:
Amino acids are converted to pyruvate, and therefore depend on PDH to be further oxidized.
Alanine (Ala)- clears toxins released during muscle protein breakdown, aids in regulating blood glucose and cholesterol, serves as an energy source for muscles and the CNS and assist in lymphocyte production
Cysteine (Cys)- stimulates collagen production and is involved in the synthesis of glutathione, and plays a role in protein folding and stability
Glycine (Gly)- is involved in the production of DNA, phospholipids and collagen. It helps in the production of bile acids and supports glutathione synthesis. It acts as an inhibitory neurotransmitter in the CNS. Aspartate and glycine form an excitatory/inhibitory pain in the spinal cord, similar to glutamate/GABA in the brain.(20)
Serine (Ser)- is involved in phospholipid formation, is a precursor of several amino acids including glycine and cysteine. It plays a role in DNA and RNA synthesis, and is important for proper functioning of the CNS.
Threonine (Thr)- is a principal part of structural proteins such as collagen and elastin, which are important components of your skin and connective tissue. It also plays a role in fat metabolism and immune function.
The amino acids needed for ATP production and utilization include: (54)
Glutamine which can be converted into glucose through gluconeogenesis, then used to produce ATP
Alanine serves as a substrate for gluconeogenesis, especially when fasting or intense exercise
Arginine plays a role in the urea cycle and can be converted into nitric oxide that is involved in various signalling pathways influencing energy metabolism, as well as contributing to the synthesis of creatine which helps in ATL production
Methionine is involved in the synthesis of S-adenosylmethionine (SAMe), a critical donor in biochemical reactions, including those affecting ATP production and energy metabolism. DNA mutations in this have consistently been seen by DNA studies by Dr Valerio Vittone.(3)
Leucine activates the mTOR pathway that promotes protein synthesis and cellular growth, indirectly supporting ATP production by enhancing muscle recovery and energy utilization
Cysteine is important for synthesizing glutathione, an anti-oxidant that protects cells from oxidative stress which can impair ATP synthesis
Glycine is involved in the synthesis of creatine, which is crucial for ATP regeneration in muscle cells.
Other amino acids potentially involving the thromboinflammatory pathway:
Arginine, a precursor for nitric oxide, which plays a role in vascular function and inflammation
Glutamine, with its role in immune function and inflammatory responses
Cysteine important for glutathione synthesis, which is crucial for antioxidant defence
Tryptophan involved in kynurenine pathway -linked to inflammation and oxidative stress
Glycine has anti-inflammatory properties and is involved in haeme synthesis
Methionine is important for methylation reactions and can affect homocysteine levels, linked to cardiovascular risk
Associated Vitamin Deficiencies
When amino acids are low, a number of vitamins may be low or in increased demand.
Vitamin B6 (pyridoxine) is important for neurotransmitter synthesis and immune function may be depleted by increased metabolic demands. It is a required cofactor for amino acid metabolism.
L dopa and tryptophan are dietary derived. Amino acid decarboxylase is also B6 dependant.
Symptoms of B6 deficiency:
Skin rashes, especially seborrhoeic dermatitis
Cracked sore lips and angle of mouth
Anaemia and fatigue
Depression and confusion
Immune dysfunction
Neurological symptoms eg neuropathic pain and numbness
Symptoms of B6 toxicity: this is almost never caused by dietary intake, but usually by high-dose supplements.
Peripheral neuropathy
Painful skin lesions
Photosensitivity
Nausea
Impaired body movements
Vitamin B12 (cobalamin) works closely with folate in amino acid metabolism and protein synthesis. The impact of PEM on B12 is not fully understood but potential impacts include:
Increased oxidative stress with breakdown of amino acids, indirectly affecting B12 metabolism and methylation reactions
Mitochondrial dysfunction. PEM involves mitochondrial dysfunction and B12 is crucial for cellular energy production, this dysfunction interfering with B12 utilization at a cellular level
Altered methylation. B12 is essential for methylation processes, and the metabolic stress in PEM may increase the demand for methylation reactions, affecting B12 utilization and availability
Impaired B12 absorption with the association with gut dysfunction in CFS
· Altered energy metabolism and potential for B12 deficiency
Folate (vitamin B9) essential for metabolism of several amino acids including histidine, glycine and serine
Vitamin C (ascorbic acid) Vitamin C, a powerful antioxidant may be affected by PEM as the body attempts to deal with the increased oxidative stress, and a higher utilization.
The “burn off” or catabolism of amino acids in PEM could indirectly affect Vitamin C dependent pathways, eg metabolism of Tyrosine.
Collagen synthesis may be affected by the PEM’s demand for vitamin C
The immune stress in PEM may increase the demand for Vitamin C to maintain immune defences
Vitamin B3 (niacin) is involved in the synthesis of tryptophan. Low niacin can affect tryptophan breakdown and serotonin production.
Vitamin B1 (thiamine) plays a role in metabolism of branched chain acids leucine, isoleucine and valine.
Vitamin D which is important for calcium absorption, immune function and neuromuscular health may be affected by altered absorption and increased utilization
Vitamin E an antioxidant protecting cell membranes may be depleted in oxidative stress
Associated Mineral Deficiencies
Zinc is essential for immune function, wound healing, DNA synthesis and is a cofactor for numerous enzymes. In a “burn off” potential mechanisms include increased demand from chronic inflammation, altered absorption or increased excretion and utilization in antioxidant processes.
Magnesium is critical for energy production, nerve function and muscle contraction. With depletion from increased metabolic demands
Selenium an antioxidant supporting immune function nand thyroid hormone metabolism may be depleted by increased oxidative stress
Copper is necessary for iron metabolism and antioxidant function, and may be altered by altered absorption and increased utilization
Manganese plays an important role in collagen formation and function and is required for the activation of prolidase an enzyme crucial for collagen synthesis
Major Neurotransmitter Pathways
The balance and interplay between these different neurotransmitter pathways are crucial for normal brain function, and disruptions can lead to neurological and psychiatric disorders.
Dopamine pathways- involved in reward, motivation and motor control. Dopamine is the primary neurotransmitter in the mesolimbic (reward) pathway, involved in motivation, pleasure and reinforcement of behaviours. Dopamine pathways are critical for motor function, with disruptions leading to Parkinson’s Disease.
Serotonin pathways- projections to various parts of the nervous system influencing functions such as sleep, memory, appetite and mood. It is involved in regulating sleep-wake cycles.
Glutamate pathways- the major excitatory system in the brain and linked to many other neurotransmitter pathways, including GABA, the main inhibitory neurotransmitter. Glutamate is involved in numerous pathways related to learning, memory and cognitive function.(18)
GABA (Gamma-aminobutyric acid) is the main inhibitory neurotransmitter in the CNS, helping to regulate and balance neural activity. GABA pathways are involved in managing anxiety and stress responses.
Acetylcholine (Ach) pathways are crucial for maintaining cognitive function, with disruptions linked to Alzheimer’s disease. In the peripheral nervous system Ach is essential for muscle activation.
Noradrenaline pathways are involved in regulating arousal, attention and fight-or flight responses. It plays a key role in the sympathetic nervous system influencing heart rate, blood pressure and other autonomic functions
Histamine pathways are involved in regulating the sleep-wake cycle and promoting wakefulness. Histamine pathways are also involved in immune responses and brain inflammation.
Conclusion
We provide a preliminary assessment of ongoing amino acid assays where brainstem hypoperfusion, coat hanger pain and PEM is present, that same group of medical conditions linked by fatigue and cognitive impairment- ME/CFS, POTS, Fibromyalgia Syndrome, Gulf War Syndrome and Long COVID. Research into coat hanger pain has provided pathways demonstrating where hypoperfusion induces muscle depolarization, weakness, and mitochondrial dysfunction.
ME/CFS patients have reduced reserves of Adenosine triphosphate (ATP) vital for mitochondrial energy production, and replenishment of ATP may take days. In post exertional malaise (PEM) the metabolic dysfunction induced by the hypoperfusion moves the body into catabolic metabolism with increased “burn off” of amino acids, essential minerals and vitamins.
The hypoxia is believed to impair the function of enzyme pyruvate dehydrogenase (PDH) in ME/CFS causing changes in the vital Krebs/ Citric acid cycle responsible for our mitochondrial energy source leading to inadequate adenosine triphosphate (ATP) generation by oxidative phosphorylation and excessive lactate generation on exertion.(1)
Glutamate metabolism is complex and interacts with many other biochemical pathways. The specific amino acid profiles found in these studies may provide clues about the nature of the dysfunction, potentially guiding treatment approaches.(42) For example, addressing amino acid imbalances or targeting glutamate receptors may be more appropriate depending on the observed pattern. These patterns highlight the heterogeneity of glutamate-related disorders and the need for personalized approaches in diagnosis and treatment. Further research is needed to fully understand the implications of these amino acid profiles and their relationship to specific symptoms or conditions.
I believe these preliminary studies will foster a more detailed research that affects ME/CFS, POTS, Fibromyalgia as well as Long COVID and lay the path for improved management protocols.
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